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Apex Taq RED Master Mix, 2X
產(chǎn)品型號(hào):Cat #: 42-138B
簡(jiǎn)介:


1.5mM MgCl2 (Final Conc.)
1000 Reactions/Unit
Brand: Apex Bioresearch Products
Apex 2X RED Taq Master Mix, 1.5mM MgCl2 [Final Conc.] with ammonium buffer that promotes robust amplific

Apex Taq RED Master Mix, 2X  的詳細(xì)介紹

Apex™ Taq Master Mixes are ready-to-use 2.0x reaction mixes. They contain Apex Taq DNA Polymerase, an Ammonium based Buffer System, Apex ultra pure dNTPs (>99%) and Magnesium Chloride. Simply add primers, template, and water to successfully carry out primer extension and other molecular biology applications. Master Mixes offer convenience and reproducibility as reactions can be set up with less pipetting steps. The number of reactions is based on using 25μl of the master mix for a final reaction volume of 50μl. If desired, the reaction size may be scaled down to a final volume of 20 or 25μl by using 10μl or 12.5μl of the master mix respectively.

Apex Taq DNA Polymerase possesses 5'-3' DNA polymerase activity and a double strand 5'-3' exonuclease activity. It leaves an 'A' overhang which makes it ideal for TA cloning. The robust nature of this enzyme means that Apex Taq can be used with little optimization.

Product Overhang 3'-dA
Hot-Start No
5' - 3' Exonuclease activity Yes
Concentration 2X
Loading Dye Yes
Storage Long term storage (3 years) at -20°C Short term storage (6 months) at 4°C
Max Amplicon Length 5kb
Composition Tris-HCl pH 8.5 (NH4)2S04 3 mM MgCl2 0.2 % Tween? 20 0.4 mM of each dNTP 0.08 units/μl Taq DNA polymerase Inert Red Loading Dye and Stabilizer
Expiration The expiration date is labeled on each individual tube and is dependent on proper long term storage conditions
Packaging Configuration 20 x 1.25ml


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